Fig. 4 | Scientific Reports

Fig. 4

From: Resolvin D1 combined with exercise rehabilitation alleviates neurological injury in mice with intracranial hemorrhage via the BDNF/TrkB/PI3K/AKT pathway

Fig. 4

RvD1 combined with exercise rehabilitation training can activate BDNF/TrκB/p-Akt/PI3K related pathway and increase its protein and mRNA expression. (a) Western blot analysis was used to detect the expression levels of BDNF, TrκB, PI3K proteins, and both phosphorylated and total Akt. (b-d) Quantitative analysis of BDNF, TrκB, and PI3K protein expressions in brain tissue adjacent to the injection site on the ipsilateral side after the experiment. (e) Semiquantitative measurements of the ratio of phosphorylated to total Akt. (f-i) Expression levels of BDNF (f), TrκB (g), PI3K (h), and Akt (i) mRNAs at the injection site were determined. Western blot and RT-qPCR analyses revealed that the expression levels of BDNF, TrκB, p-Akt, and PI3K in the ICH + RvD1 + exercise training group were significantly higher compared to those in the ICH group. (*Significant vs. ICH group, p < 0.05).

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