Table 1 Primer sequences of SSR and ISSR markers used in this study, and their information.
Primer names | Sequences (5’-3’) | Tm (°C) | Number of loci | %P | PIC |
|---|---|---|---|---|---|
SSR-CL 1059 | F: TGTCCTTCCTTTGAAACAACCTA R: GTCAAAATCATGGGAGGAGAAC | 58 | 4 | 25 | 0.05 |
SSR-CL1078 | F: TGAAGGCACTGCTCAAGTCTAAT R: CACATAAAACCAAGTTTCCAACC | 58 | 4 | 25 | 0.04 |
SSR-11,213 | F: CTGAAGATCATCATCCCAATTCC R: AAAATCTGATTGGCAACATAGGA | 57.1 | 8 | 50 | 0.13 |
SSR-10,430 | F: ATTACGTGCAGAGGCATTTGAT R: GAATCTTGAGTCTTGCTTTCCA | 56.5 | 4 | 50 | 0.14 |
SSR-CL275 | F: CAGTACGATTTCTGAATGTCACG R: TATCTTCTGCTCTGCTCTGCTCT | 59.75 | 5 | 60 | 0.11 |
SSR-6327 | R: GAGATAGAGAATTGAAGACCGCC F: GCAGAAGCAACCACTATTCTCAG | 60.6 | 5 | 20 | 0.07 |
SSR-10,310 | F: AAACAGACCACCCTTGATTTTCT R: CAGGGAATTCTCCATTACCAAAG | 58 | 6 | 50 | 0.11 |
SSR-17,583 | F: GGATGAATCAGCTTCTTCAGAGA R: GGTTGAGTGTGAACGAGTTTTGT | 58.9 | 6 | 50 | 0.06 |
SSR-10,047 | F: TTCCCCCTACTACCATTATAGCC R: TGAATGTAACAAATGTGGTGCTC | 58.85 | 4 | 40 | 0.15 |
SSR-17,585 | F: GCTTCTGCCTATGCTGTTGAGTA R: TATACCTCGAGAACTGCTTCCAC | 60.6 | 5 | 60 | 0.03 |
SSR-10,198 | F: TCTTGGAGACATAAGGCACAGAT R: GAAGAAGGAGCAAAGAACAATGA | 58 | 6 | 50 | 0.14 |
SSR-1020 | F: GCTGATCAGGTGTGGATCTGAC R: GTTTTGGGTGAGTGAATCTTCTG | 49 | 5 | 20 | 0.07 |
SSR-4211 | F: TCCTCTCCACCTCCTAAACAAAC R: CTTTTTGAAAATGTCTTTCCAGC | 57.95 | 5 | 40 | 0.06 |
SSR-6683 | F: GCAGTGAGCACATCGTTGAC R: CAGGAAAAGTTGGCTCTCAGG | 63 | 9 | 40 | 0.07 |
SSR-CL2728 | F: TCATCAAAGATGTTTCCTTCCAT R: ATAAGTTCATGTAATCGCATGGC | 56.2 | 4 | 50 | 0.06 |
SSR-16,847 | F: TCAAAGCTCAAACCTAAACCAAA R: GACACTGTTTCTACGCCATCTTC | 57.95 | 4 | 50 | 0.04 |
SSR-6277 | F: ACTTCTGCCTATGCTGTTGAGTA R: TTTTTGGGTGAGTGAATCTTCTG | 55.6 | 3 | 100 | 0.23 |
SSR-6195 | F: GCAAAGCTCAAACCTAAACCAAA R: CACACTGTTTCTACGCCATCTTC | 54.2 | 6 | 33.33 | 0.12 |
SSR-6390 | F: TGGTGAAGATTGTGATACTAGGGA R: TATCTGAGGGTTTGGACTTCTGA | 60 | 5 | 60 | 0.10 |
SSR-6357 | F: AGCACGTGCAGAGGCATTTGAT R: TCGATCTTGAGTCTTGCTTTCCA | 52.2 | 5 | 20 | 0.02 |
ISSR-17,899 A | (CA)7AG | 49.2 | 4 | 50 | 0.35 |
ISSR-17899B | (CA)7GG | 51.7 | 5 | 60 | 0.35 |
ISSR-HB8 | (GA)6GG | 44 | 6 | 100 | 0.36 |
ISSR-HB9 | (GT)6GG | 44 | 9 | 100 | 0.22 |
ISSR-HB10 | (GA)6CC | 44 | 5 | 80 | 0.22 |
ISSR-HB12 | (CAC)3GC | 38 | 4 | 25 | 0.07 |
ISSR-UBC807 | (AG)8T | 49.8 | 5 | 60 | 0.08 |
ISSR-UBC809 | (AG)8G | 52.2 | 11 | 81.81 | 0.21 |
ISSR-UBC823 | (TC)8 C | 52.2 | 4 | 25 | 0.05 |
ISSR-UBC825 | (AC)8T | 49.8 | 7 | 42.85 | 0.11 |
ISSR-UBC827 | (AC)8G | 52.2 | 7 | 57.14 | 0.17 |
ISSR-UBC841 | (GA)8YC | 52.6 | 6 | 66.66 | 0.12 |
ISSR-UBC844 | (CT)8AC | 52.6 | 6 | 100 | 0.24 |
SSR average | 5.15 | 44.66 | 0.09 | ||
ISSR average | 6.07 | 65.26 | 0.20 |
