Fig. 2 | Scientific Reports

Fig. 2

From: Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET

Fig. 2

Degradation profile of wildtype c-MET in A549 cells. (A–J) Cells were pre-treated as described in (Fig. 1) with HGF as RTK ligand and either SJF8240 (A,C,E,G,I) or Foretinib (B,D,F,H,J) for 24 h. Cells were then analysed by immunoblotting (A,B) and quantification (C,D), whole cell (E,F) or plasma membrane (G,H) immunofluorescence, or by flow cytometry (I,J). Graphs show the mean and SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. Asterisks highlight p values < 0.01. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 1).

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