Fig. 3 | Scientific Reports

Fig. 3

From: Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET

Fig. 3

Degradation profile of ex14del c-MET in HS746T cells. Cells were pre-treated as described in (Fig. 1) with HGF as RTK ligand and either SJF8240 (A,C,E,G,I) or Foretinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 2).

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