Fig. 4 | Scientific Reports

Fig. 4

From: Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET

Fig. 4

Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).

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