Fig. 4

Light scattering experiments with FtsZ1-2 and FtsZ2-1, individually and in combination of 5 mM. Individual light scattering experiments were performed using 1.875 µM, 3.5 µM, 7.5 µM or 15 µM of either of FtsZ1-2 in buffer C (A) or FtsZ2-1 in buffer B (B). The combined light scattering experiments were performed in a buffer containing 37.5 mM glycine (pH 10), 100 mM KCl, 5 mM MgCl₂, and 5% glycerol. For these experiments, 7.5 µM of each FtsZ1-2 and FtsZ2-1 were mixed and analysed in technical triplicates. Additionally, 7.5 µM of each FtsZ variant was measured separately in triplicates under the same buffer conditions (C). Light scattering in counts per seconds (cps).