Fig. 5

GTPase activity of Physcomitrella FtsZ1-2 and FtsZ2-1, individually and in combination. GTPase assays were performed at 25 °C using either 7.5 µM of FtsZ1-2 or 7.5 µM of FtsZ2-1 individually, or a mixture of 7.5 µM FtsZ1-2 with 7.5 µM FtsZ2-1. The assays were performed in a buffer containing 37.5 mM glycine (pH 10), 100 mM KCl, 5 mM MgCl₂, and 5% glycerol in the presence of 1 mM GTP and conducted in technical triplicates. To account for background GTP auto-hydrolysis, parallel reactions without FtsZ variants were included for each replicate. Background values were subtracted from the corresponding experimental measurements. Absorbance was measured at 620 nm after a 10-minute incubation. The amount of free phosphate (µM) released per minute was determined using a phosphate standard calibration curve. Data are presented as mean ± standard deviation from three technical replicates.