Table 2 Morphological and molecular characteristics of marine endophytic fungi associated with Sargassum Sp.
Isolate | SE-1 | SE-2 | SE-3 | |
|---|---|---|---|---|
Identified fungal taxon | Chaetomium globosum | Aspergillus terreus | Curvularia perotidis | |
Macroscopic characteristics* | ||||
Growth rate | Rapid | Rapid | Slow | |
Diameter after 6 days (approx.) | 8.5–9.0 cm | 8.5–9.0 cm | 1.7–2.0 cm | |
Hyphae | Aerial, surficial, submerged | Surficial, submerged | Surficial, submerged | |
Mycelium depth in agar | Shallow | Shallow | Shallow | |
Form of colony | Filamentous | Filamentous | Irregular | |
Colour of colony surface | White initially, becoming greyish olivaceous | White initially, then yellow, finally cinnamon brown | Pale yellow initially, then brownish-yellow | |
Colour of colony reverse | Yellowish brown to dark brown | Yellowish-brown | Reddish-yellow, becoming darker with age | |
Texture of colony surface | Cottony | Powdery | Granular with woolly edge | |
Elevation of colony | Crateriform | Raised | Flat | |
Margin of colony | Filiform | Filiform | Undulate | |
Microscopic characteristics* | ||||
Spore view at | 28 days | 3 days | 12 days | |
Hyphae | Septate | Septate | Septate, dark | |
Conidiophores | Large, oval shaped, brown, fragile with wavy ascomatal hairs; asci stalked, club shaped | Unbranched, relatively short, smooth, hyaline; conidial heads biseriate, covering the upper half of vesicle, compactly columnar | Branched, mostly bifurcated; conidiogenous cells discrete, subcylindrical | |
Conidia | Lemon shaped, olive-brown, single celled | Round shaped, hyaline, single celled | Straight or very-slightly curved, pale brown, multicellular | |
Molecular characteristics | ||||
BLAST (ITS sequence) | Total score | 887 | 1110 | 279 |
Query cover/ Identity (%) | 98/ 98.99 | 96/ 100 | 65/ 93.16 | |
Bootstrap support value (%) | 77 | 78 | 53 | |
GenBank accession no. | OR338812 | OR336320 | OR335556 | |
