Fig. 2 | Scientific Reports

Fig. 2

From: Restorative potential of ciliary body cells in a retinal ganglion cell degeneration model

Fig. 2

CB, NS-CBC and NS SVZ survive long-term after intravitreal administration, do not integrate into the retina and are not detrimental to neurons in the ganglion cell layer. (A) Design of the primary ciliary body cells (CB), collection and cultivation process to form neurosphere-expanded CB (Ns-CB) and the obtention of neural stem cells from the subventricular zone (Ns-SVZ). (B) CB, Ns-CB or Ns-SVZ of C57/BL/6 Tg(CAG-EGFP) mice were intravitreally administered in intact retinas (toxicity analysis) of C57BL/6 mice. (C) Confocal images taken from the vitreous side of flat mounts of untreated and intravitreally transplanted intact retinas 90 days after the transplant. Transplanted cells (green) form an epimembrane above the ganglion cell layer (DAPI staining) without integrating into the retina (bottom row). (D) Scatter bar graph showing the mean total number ± SD of DAPI nuclei in the ganglion cell layer. (E) Scatter bar graph showing the mean total number ± SD Brn3a*RGCs in untreated and intravitreally transplanted intact retinas analysed at 45 and 90 days after transplantation (One-way ANOVA, Tukey’s multiple comparisons test). Data from one independent experiment. (F) Neighbour maps of untreated and intravitreally transplanted intact retinas analysed 90 days after the transplant showing the topographical distribution of Brn3a+ RGCs. Colour scale goes from 0–22 (purple) to ≥ 160 (red) neighbour RGCs in a radius of 0.11 mm. The bottom-right of each panel shows the number of RGCs counted in its corresponding retina. S: superior, N nasal.

Back to article page