Fig. 4

miR-7-5p/PKP2 axis regulates radiosensitivity via DNA damage and NHEJ repair. (A,B) Clonal survival of A549 cells after 6 Gy IR. Data are shown as mean ± SD from a Student’s t-test. (C) Cell viability after 6 Gy IR. Data are shown as mean ± SEM from three independent experiments. (D,E) IF staining of H2AX foci in the indicated cells after exposure to 6 Gy IR. Scale bar = 20 μm. Data are shown as mean ± SD from a Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001. (F,G) Detection of NHEJ activity in EJ5-GFP cells. Cells were transfected with I-SceI and miR-NC, miR-7-5p mimic, or wild-type PKP2 plasmids by electroporation, then analyzed by flow cytometry (F) and quantified (G). Data are shown as mean ± SEM from three independent trials.