Fig. 1
Experimental setup, SEM analysis and the viability of MSCs/β-TCP after water medium irrigation. (A) A SECCS system was used to prepare β-TCP bone material enriched with bone marrow MSCs (MSCs/β-TCP), and the experimental setup for water medium irrigation with MSCs/β-TCP is shown. (B) SEM images of β-TCP alone (NC), enriched MSC/β-TCP(PC/0), MSC/β-TCP particles after irrigation for different durations (5, 15, 30, and 60 min), arrows showing MSC cell adhesion and surface morphology. The scale bars represent 5 μm in the top images, 20 μm in the middle images, and 100 μm in the bottom images. (C) Results of the CCK-8 assay of MSC/β-TCP particles after irrigation for different durations (0, 5, 15, 30, and 60 min), showing cell viability (n = 3). *P < 0.05 versus the 0-min group, #P < 0.05 versus the 30-min group.
