Fig. 2
Impact of irrigation duration on the biological activities of the MSC/β-TCP composites. (A,B) ALP staining of MSCs/β-TCP at various irrigation times (0, 5, 15, 30, and 60 min) and quantitative analysis of ALP staining (n = 6). (C,D) Alizarin Red S staining of MSCs/β-TCP at various irrigation times (0, 5, 15, 30, and 60 min) and quantitative analysis of Alizarin Red S staining (n = 6). (E,F) Oil Red O staining of MSCs/β-TCP at various irrigation times (0, 5, 15, 30, and 60 min) and quantitative analysis of Oil Red O staining (n = 6). (G) Toluidine blue staining of MSC/β-TCP particles after irrigation for different durations (0, 5, 15 and 30 min) revealed reduced staining intensity (n = 6). *P < 0.05 versus the 0-min group, #P < 0.05 versus the 30-min group.
