Fig. 5
From: DRP2 promotes EMT and serves as a potential therapeutic target for LUAD treatment
Knockdown of DRP2 inhibits proliferation in LUAD cells. (A–C) Cell cycle distribution was analyzed by flow cytometry. The percentage of cell number in each phase was calculated. (D.E) Proliferation of DRP2 knockdown and control cells was detected, and the percentage of Edu-positive nuclei was counted after 48 h of culture.Scale bar = 100 μm. (F) CCK8 analysis was used to detect the cell viability of knockdown and control DRP2 cells. (G,H) A549 and H1299 cells depleted of DRP2 (sh-NC as a negative control) were subjected to colony formation assays. Representative images were shown in (G). Colonies were quantified in (H). Each experiment was performed three times and the data were expressed as mean ± standard deviation (SD, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus “sh-NC” group.
