Fig. 2
From: A novel mouse model for X-linked Alport syndrome induced by splicing mutation in the Col4a5 gene
Minigene validation experiment for the splicing pattern of the Col4a5-c.1517-1G > T mutation. (A) Minigene Experimental Workflow: Before the experiment, the contiguous intronic sequences of exons 22 to 24 of the Col4a5 gene, featuring the c.1517-1G mutation, and the WT control were individually cloned into the vector. The Col4a5-c.1517-1G > T mutation leads to skipping of exon 23, resulting in an amplified fragment length of 403 bp. (B) Sequencing results of the wild-type and Col4a5-c.1517-1G > T type transcripts.
