Fig. 3
From: A novel mouse model for X-linked Alport syndrome induced by splicing mutation in the Col4a5 gene
Targeting strategy, genotyping, and clinical phenotypes of Col4a5-c.1517-1G > T mutant mice. (A) The target locus was genetically modified through homologous recombination in embryonic stem cells using the CRISPR/Cas9 system. (B) Electrophoresis results of the PCR products in the target region of F1 mice. Number 126, 127, 128 refer to KI mice, while number 129 and C57L/B6J are chose as control. (C) Sequencing results of the target region in F1 mice. The red mark indicates the Col4a5-c.1517-1G > T mutation site.
