Fig. 5
From: A novel mouse model for X-linked Alport syndrome induced by splicing mutation in the Col4a5 gene
Pathological changes in the kidney tissue of Col4a5-c.1517-1G > T mutant mice. (A-C) Representative images of H&E(A), PAS(B), and Masson(C) staining of kidney tissue in wild-type and KI mice at 7 weeks, 14 weeks, 21 weeks, and 28 weeks of age (H&E staining magnification ×100, other staining magnification ×200). The yellow arrows indicate infiltration of mononuclear cells (A). The black box highlights abnormal thickening of the glomerular basement membrane, glomerular atrophy, and sclerosis (B). The green arrows represent deposition of collagen fibers, with focal fibrosis present in both the glomeruli and renal tubules (C). (D) Quantification of podocytes in wild-type and KI groups from 7 to 28 weeks, showing a progressive decrease in podocyte number in the KI group compared to the control group. The average podocyte density per glomerulus is the number of WT1 positive cells/number of glomeruli. P < 0.01 **, unpaired t-test was used for inter-group comparison.
