Fig. 1
From: Enhanced application potential of alveolar organoids through epithelial and niche cell interactions
Alveolar organoids (AOs) generation and characterization. (A) Schematic overview of the alveolar organoids (AOs) generating protocol for human lung tissue derived cells. (B) Representative brightfield image of 2D progenitor cells (top left and right) and 3D AOs at different stages of differentiation, early (bottom left) and late (bottom right); scale bar: notation for each. (C) In mature AOs, the expression of alveolar epithelial cell-specific proteins for type 1 (AT1) and type 2 (AT2) cells was examined using immunostaining analysis; scale bar: 100 μm. (D) Western blot analysis for expression of alveolar region-specific cell markers in mature AOs from each of four different donors; n = 4. (E) Immunostaining analysis for expression of niche cell markers in AOs; scale bar: notation for each. (F) Compare the percentage of cellular composition of AOs from different donors evaluated by FACS.
