Fig. 2
From: Enhanced application potential of alveolar organoids through epithelial and niche cell interactions
Comparison organoid formation ability between sorted and non-sorted cells. (A) Ratio of cell composition of the LysoTracker-positive cell (LT) and the HTII-280-positive cell (AT2); n = 5. (B) Ratio of cell composition in LT-positive cell; LT + HTII-280 + = red bar and LT + HTII-280- = gray bar; n = 5. (C) Immunostaining analysis comparing the AT1 (HTI-56, Aquaporin-5) and A2 (SPC, HTII-280) between non-sorted and AT2-LT + sorted cells. Fluorescence images were captured using a confocal microscope; scale bar: 100 μm. (D) Comparison of expression of alveolar niche cell markers (PDGFR⍺, FGF10) between non-sorted cells and AT2-LT + sorted cells using immunostaining analysis.; scale bar: 100 μm. (E) Immunostaining analysis comparing the expression of AT1 (PDPN) and niche cell (FGF10) between non-sorted and AT2-LT + sorted cells; scale bar: 20 μm and Three-dimensional visualization of the immune-stained structure from bottom of E, shown from a different angle to highlight the spatial organization of PDPN and FGF10 expression in alveolar epithelial cells and niche cells.
