Fig. 3

Population structure in samples from 2022 rainy season. A) Principal Coordinates Analysis with allele frequencies. Colors indicate administrative regions (red tones, North; green tones, Centre; blue tones, South). Samples with mutations in pfdhp-s436 and pfdhps-581 codons are indicated with hollow shapes. Bray-Curtis was used as the dissimilarity metric. B) Permutational Mantel test of Bray-Curtis distance calculated from population allele frequencies vs. geographic distance (Mantel’s rho = 0.73 p = 0.001; Spearman’s test with 10000 permutations).