Fig. 4
Microglial response in the FC and the OB at 24H and 28D. (A) Microglial density quantification in the FC at 24h and 28 days post-exposure in number of marked cells per mm2 ± SEM. n = 4–5 per group. (B) Illustration of microglia stained with a microglial marker (IBA1, green) and DAPI (blue) in the apex of the FC. (C) Illustration of microglia stained with a microglial marker with (IBA1, green) and DAPI (blue) in the granular layer of OB. (D) Microglial density scoring in the OB at 24h and 28 days post-exposure ± SEM. Arbitrary units (a.u.). n = 5 per group. (E) Microglial activation scoring in the OB 24h and 28 days post-exposure ± SEM. Arbitrary units (a.u.). n = 5 per group. Results passed normality tests for figures A and D and were analyzed using One-way ANOVA. Results for figure E did not pass the normality test and were analyzed using a Kruskal–Wallis test. All groups were compared to each other within each time point. Significant differences between groups are indicated by asterisks (*p < 0.05; **p < 0.01).
