Fig. 5
Inflammation and antioxidant response linked gene and protein expression in OB at 24h and 28 days post-exposure. (A) Gene expression levels of Sod2, Catalase, Tnfα and Mcp1 in the OB at 24h post-exposure ± SEM. Arbitrary units (a.u.). n = 7–8 per group. (B) Gene expression levels of Sod2, Catalase, Tnfα and Mcp1 in the OB at 28 days post-exposure ± SEM. Arbitrary units (a.u.). n = 7–8 per group. (C) OB 24h post-exposure protein expression levels of NRF2 and pellet/supernatant ratio of NRF2 to assess nuclear translocation ± SEM. Arbitrary units (a.u.). n = 5–6 per group. (D) OB 28 days post-exposure protein expression levels of NRF2 and pellet/supernatant ratio of NRF2 to assess nuclear translocation ± SEM. Arbitrary units (a.u.). n = 6 per group. (E, F, G) Representative Western blot membranes used to quantify the protein expression of NRF2 and normalize with GAPDH (whole cell lysate or cytosolic fraction) or β-actin (nuclear fraction). Western blot membranes images were cropped (E to G) and original blots are presented in Supplementary Figure 2 (Sup. Figure 2E to G). Western blots were done in duplicates. All results passed normality tests were analyzed using a One-way ANOVA. All groups were compared to each other within each time point. Significant differences between groups are indicated by asterisks (*p < 0.05; **p < 0.01).
