Fig. 3

Optimization and validation of ssDNA reporter volume for visual detection: (a) A, B, and C are three groups of replicate experiments. 1, 0.66 µL; 2, 0.99 µL; 3, 1.32 µL; 4, 1.65 µL; and 5, negative control. (b) Fluorescence quantification results for different reporter volumes.