Fig. 5

Sensitivity evaluation of the RPA-CRISPR/Cas12b visual detection system: (a) A, B, C, D, E and F are six groups of replicate experiments. 1, 10 ng µL− 1; 2, 2 ng µL− 1; 3, 0.4 ng µL− 1; 4, 0.08 ng µL− 1; 5, 0.016 ng µL− 1; 6, 3.2 pg µL− 1; 7, 0.64 pg µL− 1; and 8, negative controls. (b) Fluorescence quantification results for different gene concentration.