Table 1 Detailed primer sequences for RPA, gRNA, and SsDNA reporter.

From: Rapid visual detection assay for Bactrocera dorsalis (Hendel) using recombinase polymerase amplification and CRISPR/Cas12b

Primer name

Sequence (5‘-3’)

RPA-F

TAACAGCTTTATTACTTTTATTATCATTAC

RPA-R

TCCTCCTCCGGCAGGGTCAAAAAAGGAAGT

gRNA

GUCUAAAGGACAGAUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGCCCGUUGAACUUCAAGCGAAGUGGCACCUAACAGACCGAAACUUAAA

ssDNA reporter

FAM-TTTTTTT-BHQ1

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