Fig. 1
From: Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells
Schematic representation of protein microarray and data analysis. MEG-01 cell lysates collected from uninfected or DENV2-infected (MOI of 5, at 72Â h p.i.) groups were mixed with lysis beads in extraction buffer. Following centrifugation step, beads were removed, and clear supernatants were collected and purified. Protein lysates were estimated for amounts by BCA assay and by using the Cytation 7 reader. Purified protein samples were biotinylated and added onto pre-blocked antibody-coated microarray slides (with 6 replicates for each antibody, and 64 spots in total). After coupling reaction, Cy3-tagged streptavidin was added onto the microarray slide to perform the hybridization reaction. After several rounds of washing, images were acquired using a GenePix microarray scanner. The scanned images were analyzed using GenePix Pro software from Full Moon Bio-Systems. The schematics is created in Bio Render.com.
