Fig. 5
From: Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells
DENV2 infection upregulates CDK4, CDK1 and Cyclin B1 transcripts and protein levels. QRT-PCR analysis showing DENV2 capsid transcript levels (A), or CDK4 (B), CDK1 (C) and Cyclin B1 (D) gene expression in DENV2-infected (MOI of 5, at 72 h p.i.) or uninfected MEG-01 cells. DENV2-loads and mRNA expression levels of CDK4, CDK1 and Cyclin B1 genes were normalized to human beta-actin mRNA levels. Y-axis represents levels of transcript and X-axis indicates uninfected or DENV2-infected groups. P value was determined by Student’s two-tail-test is shown. Each circle or square indicates one data value and n = 6 (six independent replicates run in duplicate for QRT-PCR). Immunoblotting analyses (E–J) performed using cell lysates generated from uninfected or DENV2-infected (MOI of 5, at 72 h p.i.) MEG-01 cells are shown. Immunoblot images showing DENV2-capsid protein loads (E), or CDK4 (F), Phospho-CDK4 (G), CDK1 (H), Cyclin B1 (I) and actin (J) protein loads from uninfected or DENV2-infceted samples. Total protein profile images serve as loading controls for respective immunoblots.
