Table 5 Effect of various dietary fat applications in broiler chicken diets on the breast muscle fatty acid profile (g/100 g FA).

Component	Treatment								⁹SEM	P value
Component	¹HI	²SO	³RO	⁴PO	⁵PKFD	⁶PF	⁷PL	⁸BT	⁹SEM	P value
Saturated fatty acids
C12:0	0.18	0.18	0.19	0.19	0.18	0.18	0.18	0.18	0.001	0.733
C14:0	0.96	0.94	0.96	0.96	0.94	0.93	0.94	0.94	0.003	0.311
C15:0	0.15	0.14	0.14	0.14	0.14	0.13	0.14	0.13	0.001	0.128
C16:0	15.9	16.2	15.6	15.5	16.7	16.6	14.7*	16.1	0.116	< 0.001
C17:0	0.13	0.13	0.13	0.13	0.13	0.13	0.14	0.12	0.002	0.243
C18:0	4.93	5.08	4.87	5.31	4.92	5.30	5.08	5.05	0.068	0.662
C20:0	0.19	0.18	0.19	0.18	0.19	0.19	0.18	0.18	0.001	0.103
Unsaturated fatty acids
C16:1 n7	1.39	1.39	1.42	1.41	1.40	1.39	1.38	1.41	0.007	0.775
C17:1	1.51	1.49	1.49	1.51	1.50	1.51	1.49	1.48	0.006	0.893
C18:1 n9	34.7	34.9	35.2	35.0	34.8	34.7	35.5*	34.5	0.071	0.029
C20:1	0.08	0.08	0.08	0.09	0.09	0.09	0.08	0.09	0.001	0.099
C18:2 n6	37.0	37.2	37.0	37.0	36.8	36.6	36.9	37.0	0.062	0.534
C18:3 n3	2.34	1.63*	2.27	2.24	1.76	1.71	2.86	2.28	0.071	< 0.001
C18:3 n6	0.34	0.35	0.35	0.34	0.35	0.35	0.34	0.34	0.001	0.606
C20:2	0.17	0.16	0.15	0.14	0.14	0.16	0.15	0.16	0.004	0.280
Summarized fatty acid
¹⁰SFA	22.4	22.8	22.1	22.4	23.2	23.4*	21.3*	22.7	0.110	< 0.001
¹¹UFA	77.6	77.2	77.9	77.6	76.8	76.6*	78.7*	77.3	0.110	< 0.001
¹²MUFA	37.7	37.9	38.2	38.0	37.8	37.7	38.4*	37.5	0.071	0.027
¹³PUFA	39.9	39.3	39.7	39.6	39.0*	38.9**	40.3	39.8	0.085	< 0.001
PUFA/SFA	1.78	1.72	1.80	1.77	1.69	1.66*	1.89*	1.76	0.012	< 0.001
n6	37.4	37.5	37.3	37.3	37.1	37.0	37.3	37.4	0.062	0.548
n3	2.34	1.63*	2.27	2.24	1.76	1.71	2.86	2.28	0.071	< 0.001
n6/n3	16.0	33.6	16.5	16.6	50.0*	23.8	13.8	16.5	3.198	0.002
¹⁴AI	0.257	0.262	0.252	0.252	0.269	0.268	0.237*	0.260	0.002	< 0.001
¹⁵TI	0.489	0.524	0.480	0.490	0.528*	0.538**	0.446*	0.499	0.005	< 0.001

*p < 0.05, **p < 0.01, ***p < 0.001—the reference group (BSFL) is significantly different within a row when asterisks are present. ¹BSFL—basal diet with 100% black soldier fly (H. illucens) larval fat; ²SO—basal diet with 100% soybean oil; ³RO—basal diet with 100% rapeseed oil; ⁴PO—basal diet with 100% palm oil; ⁵PKFD—basal diet with 100% palm kernel fat distillate; ⁶PF—basal diet with 100% poultry fat; ⁷PL—basal diet with 100% pig lard; ⁸BT—basal diet with 100% beef tallow; ⁹SEM—standard error of the mean; ¹⁰SFA—saturated fatty acids; ¹¹UFA—unsaturated fatty acids; ¹²MUFA—monounsaturated fatty acids; ¹³PUFA—polyunsaturated fatty acids; ¹⁴AI—atherogenic index; ¹⁵TI—thrombogenic index; Means represent 1 bird randomly chosen from each pen (n = 10).
Analysis of variance (ANOVA) or the Kruskal‒Wallis test, depending on the normal distribution of the data, provides the probability level (P value) exhibited in the last column.

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