Fig. 2 | Scientific Reports

Fig. 2

From: Ultrasound-induced mechanical damage of cancer cell cytoskeleton causes disruption of nuclear envelope and activation of cGAS-STING

Fig. 2

(A) Counts (percentage) of the indicated cells after treatment with LITUS (1 MHz, CW 20 min, 0.1 W/cm2) compared to untreated (NT) cells. Each point represents one individual experiment. (B) Left: representative images of colony-formation assays of the indicated cell lines treated or not with LITUS (1 MHz, CW 20 min, 0.1 W/cm2) and then grown for 12 days. Colonies were counted after crystal violet staining. Right: number of colonies counted in each well. *p < 0.05; **p < 0.01; each dot represents a different experiment. (C) Left: Representative confocal immunofluorescent images of the indicated cell lines treated or not with LITUS (1 MHz, CW 20 min, 0.1 W/cm2). Soon after sonication cells were fixed and stained with anti-Tubulin antibody (green signal) and DAPI (blue signal). Arrows indicate cells presented at higher magnification in Supplementary information (Fig. S5A). Right: Cells showing abnormal Tubulin signal were counted and represented in the bar graph as the percentage of the total number of cells in each field. Each dot represents a different field. 3 independent experiments were performed. Scale bar 10 μm. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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