Fig. 4

PCSK9 inhibitor improved DOX-induced myocardial apoptosis. A TUNEL staining images of myocardial tissues of the three groups of mice (n = 6), scale bar = 100 μm. B The percentage of TUNEL positive in the three groups of mice (n = 6). C Immumohis tochemical staining images of PCSK9 and Caspase-3 (n = 8), scale bar = 50 μm. D WB images of PCSK9 and apoptosis-related protein expression in myocardial tissue of the three groups of mice (n = 5). E–G Results of gray-scale scanning analysis of relative protein expression of PCSK9/GAPDH, Bax/Bcl-2 and Cleaved Caspase-3/Pro Caspase-3  (n = 5). Data are presented as mean ± SD, **P < 0.01, ***P < 0.001 vs. Control group, ^##P < 0.01, ^###P < 0.001 vs. DOX group.