Fig. 5

The changes of PCSK9 gene and protein and the localization of PCSK9 in H9C2 cardiomyocytes after DOX treatment. A The cells were treated with DOX for 12 h, 24 h and 48 h (n = 6). B Changes in relative expression of PCSK9 mRNA after DOX treatment (n = 6). C WB images of PCSK9 protein expression after DOX treatment (n = 6). D Results of gray-scale scanning analysis of relative protein expression of PCSK9/GAPDH after DOX treatment (n = 6). E Ultra-high resolution images of changes in PCSK9 localization after 24 h and 48 h of DOX treatment (n = 5), scale = 10 μm. Data are presented as mean ± SD, **P < 0.01, ***P < 0.001 vs. 0µM group, ^#P < 0.05, ^##P < 0.01 vs. 24 h control group, ^&&P < 0.01, ^&&&P < 0.001 vs. 48 h control group.