Fig. 6

Interfering with PCSK9 improved DOX-induced apoptosis of H9C2 cardiomyocytes. A WB images of apoptosis-related protein expression after DOX treatment (n = 6). B Results of gray-scale scanning analysis of relative protein expression of Cleaved Caspase-3/Pro Caspase-3 after DOX treatment (n = 6). C Results of gray-scale scanning analysis of relative protein expression of Bax/Bcl-2 after DOX treatment (n = 6). D WB images of PCSK9 gene interference effect after DOX treatment (n = 6). E Results of gray-scale scanning analysis of relative protein expression interference PCSK9 gene after DOX treatment (n = 6). F TUNEL staining images of cells treated with DOX at different times (n = 6), scale bar = 100 μm. G Percentage of TUNEL positive cells treated with DOX at different times (n = 6). H TUNEL staining showed that PCSK9 interference improved DOX-induced apoptosis in H9C2 cardiomyocytes (n = 6), scale bar = 50 μm. I Percentage statistics of apoptosis after PCSK9 interference (n = 6). Data are presented as mean ± SD, *P < 0.05 vs. 12 h control group, **P < 0.01, ***P < 0.001 vs. si-NC group, ^##P < 0.01, ^###P < 0.001 vs. 24 h control group, ^&&&P < 0.001 vs. 48 h control group.