Fig. 7

The mechanism of PCSK9 in DOX-induced apoptosis of H9C2 cardiomyocytes. A CO-IP forward verification (n = 5). B CO-IP reverse verification (n = 5), whole protein sample of H9C2 cardiomyocytes in Input group (positive control), Nonspecific IgG-binding proteins in IgG group (negative control). C WB images of KPNB1 protein expression after DOX treatment (n = 6). D Results of gray-scale scanning analysis of relative protein expression of KPNB1 after DOX treatment (n = 6). E WB images of KPNB1 and apoptosis-related proteins after interfering with PCSK9 (n = 5). F Results of gray-scale scanning analysis of relative protein expression of PCSK9, KPNB1, Bax/Bcl-2 and Cleaved Caspase-3/Pro Caspase-3 after interfering PCSK9 gene (n = 5). Data are presented as mean ± SD, *P < 0.05 vs. 12 h control group, **P < 0.01, ***P < 0.001 vs. si-NC group, ^##P < 0.01 vs. 24 h control group, ^&&P < 0.01 vs. 48 h control group.