Fig. 5

Cell osteogenesis induced by PLA-0.7. (A) Immunofluorescence staining of RUNX2 protein in BMSCs cultured with PLA-0.7 leachate medium-based osteogenesis induction medium for 24 h. (B) Relative quantification of the RUNX2 expression by immunofluorescence. Significant to control: ^***P < 0.001. (C) After co-cultured on PLA-0.7 for 1 and 2 w, BMSCs were stained with ALP and photographed by stereomicroscope. (D) After co-cultured on PLA-0.7 for 1 and 2 w, the ALP activity of BMSCs was quantitatively detected. Significant to control: ^*P < 0.05; NS: not significant. (E) BMSCs co-cultured with PLA-0.7 leachate medium-based osteogenesis induction medium for 3 w, followed by ARS staining and stereomicroscopy, and (F) semi-quantification of the calcium nodules. Significant to control: ^*P < 0.05.