Fig. 4

hUC-MSCs released histamine-induced inflammatory injury and apoptosis in the FHs 74 Int cells. (A) The cell viability of FHs 74 Int cells stimulated by different concentration of histamine. FHs 74 Int cells were stimulated with histamine (100umol/L) cocultured in the absence or presence of hUC-MSCs for 24 h. Inflammatory cytokines of (B) TNF-α, (C) IL-1β, and (D) IL-6 in the supernate of cultured cells in different groups with ELISA. (E) Representative flow cytometry plots showing apoptotic cells in different groups (Q4, Annexin V-/7-AAD-), early apoptotic cells (Q3, Annexin V +/7-AAD-), late apoptotic/necrotic cells (Q2, Annexin V +/7-AAD +), and necrotic/damaged cells (Q1, Annexin V-/7-AAD +). (F) The rate of total apoptosis (Q1 + Q2 + Q3). Data are mean ± SD (n = 3); (G) MitoSOX green staining with fluorescence microscopy. Scale bar = 50um. A: Cell viability was measured by Cell Counting Kit-8; E–F: apoptosis was measured by Annexin V-PE apoptosis Detection. * p < 0.05, **p < 0.01, ***p < 0.001, ****p = 0.000.