Fig. 3 | Scientific Reports

Fig. 3

From: Characteristic gene expression profile of intestinal mucosa early in life promotes bacterial colonization leading to healthy development of the intestinal environment

Fig. 3

Effect of age-unmatched gut microbiome transfer on bacterial compositions in young germ-free mice. (A) In cohort C, fecal samples were collected from specific pathogen-free (SPF) mice at 4 weeks of age (SPF C group), and fecal microbiota transfer (FMT) was performed in age and sex-matched germ-free (GF) mice at 4 weeks of age (ex-germ-free [exGF] C group). In cohort D, fecal samples were collected from SPF mice at 10 weeks of age (SPF D group), and FMT was performed in age-unmatched and sex-matched GF mice at 4 weeks of age (exGF D group) (n = 5 per group). Fecal samples were collected at week 0, 2, and 4. Mice were sacrificed and tissues were collected at week 4. (B) Shannon diversity index of SPF C and SPF D groups at week 0. (C) Temporal changes in the bacterial compositions of cohorts C and D. Principal coordinate analysis plots based on unweighted and weighted UniFrac distances are shown for cohort C (SPF C and exGF C groups) and cohort D (SPF D and exGF D groups). (D) Shannon diversity index of exGF C and exGF D groups at weeks 2 and 4 after FMT. Data are described as the mean ± SEM (n = 5). Male data are shown in Fig. S6.

Back to article page