Table 2 List of primers used for the quantitative analysis of immune-related gene expression in tilapia. Primer sequences, target genes, amplicon sizes, and references are provided.
Gene | Sequence (5′-3′) | GB. accession no. | Annealing temp. (°C) | R2 | Efficiency (%) | A. size (bp) |
|---|---|---|---|---|---|---|
β-actin | F: CAGCAAGCAGGAGTACGATGAG R: TGTGTGGTGTGTGGTTGTTTTG | XM_003455949.2 | 62 | 0.994 | 19.2 | 136 |
Interleukin-1β (IL-1β) | F: CAAGGATGACGACAAGCCAACC R: AGCGGACAGACATGAGAGTGC | XM_019365844.2 | 60 | 0.991 | 96.66 | 149 |
Interleukin-IL1 | F: CTGTGAAGGCATGGGTGTGGAG R: TCGCAGTGGGAGTTGGGAAG | NM_001279704.1 | 60 | 0.979 | 96.49 | 111 |
complement component (IC3) | F: GGTGTGGATGCACCTGAGAA R: GGGAAATCGGTACTTGGCCT | XM_013274267.2 | 60 | 0.998 | 97.22 | 196 |
Heat shock protein 70 (Hsp70) | F: CATCGCCTACGGTCTGGACAA R: TGCCGTCTTCAATGGTCAGGAT | FJ207463.1 | 62 | 0.995 | 99.15 | 238 |
Tumor necrosis factor- α (TNF-α) | F: AAGCCAAGGCAGCCATCCAT R: TTGACCATTCCTCCACTCCAGA | NM_001279533.1 | 58 | 0.993 | 98.67 | 184 |
Interferon (IFN-γ) | F: TGGGTGGTGTTTTGGAGTCG R: TAGCGAGCCTGAGTTGTTGG | NM_001287402.1 | 60 | 0.998 | 99.54 | 138 |
Growth factor (GF1) | F-AAGGGAAGCAGCAGCAGTTGTG R-CGTCCATGCCGTTAGCCTTGAG | NM_001279708.4 | 58 | 0.989 | 98.96 | 151 |
Growth hormone (GH) | F: ACATCATCAGCCCGATCGAC R: TCAGCAGCAAGATTCCCGTT | XM_003442542.5 | 62 | 0.994 | 98.38 | 183 |
Lysozyme (Lyz) | F-AGGGAAGCAGCAGCAGTTGTG R-CGTCCATGCCGTTAGCCTTGAG | XM_003460550.2 | 60 | 0.997 | 94.98 | 107 |
