Fig. 7 | Scientific Reports

Fig. 7

From: Pharmacokinetics of midazolam and hepatic cytochrome P450 3A activity in an in vivo extracorporeal membrane oxygenation rat model

Fig. 7

Changes in CYP3A protein expression and testosterone 6β-hydroxylation activity induced by ECMO in rat livers. Hepatic microsomal protein is isolated from the livers of the rats. (a) Testosterone 6β-hydroxylation activity is determined using the high-performance liquid chromatography‒ultraviolet method as described in the Materials and methods section. The values represent the means ± standard deviations for three rats. Statistical significance is assessed using the Kruskal–Wallis nonparametric analysis of variance test. (b) Western blotting analysis and Coomassie Brilliant Blue R-250 staining are performed. Microsomal protein (2.5 μg) is subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and assayed for CYP3A apoprotein using antibodies against rat CYP3A, as described in the Materials and methods section. The values are expressed as percentages of the sham group and represent the means ± standard deviations of three rats.

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