Fig. 5 | Scientific Reports

Fig. 5

From: TropD-detector a CRISPR/LbCas12a-based system for rapid screening of Trypanosoma cruzi in Chagas vectors and reservoirs

Fig. 5

A–C Representation of the Cytb, SR18S, and H2 A genes selected for the identification of T. cruzi, along with specific primers and gRNAs design. D Fluorescence intensity graph displaying raw data for each designed gRNA. No DNA was added to the Control. The Cytb gRNA showed significant fluorescence with p < 0.05 E Comparative graph of normalized fluorescence (Raw data/Control) corresponding to three T. cruzi samples. The culture sample demonstrated a significant difference from T. cruzi extracted from the vector’s intestine (p = 0,000189) and the reservoir (p = 0,024). F Cleavage evaluation of diluted amplified products, two bands were observed with 481 pb and 241 pb. Full gel image can be observed in the Annex Fig. 5A

Back to article page