Fig. 3
From: Single nucleus RNA-seq reveals the process from onset to chronic kidney disease in IgA nephropathy
Refined annotation of mesenchymal stromal cell subsets. (A) The UMAP plot showed the original annotation of the MSC1 and MSC2 subsets on the newly generated UMAP after re-clustering the cells from these two populations. (B) The UMAP plot displayed the more refined and precise cell type annotations on the same embedding following the re-clustering and re-annotation process. (C) The violin plot presented the expression of canonical marker genes used for precise mesenchymal stromal cell identity annotation. (D) The plot showed the top 5 highly expressed regulons in Mes (mesangial cells), Mes-Fib, SMC1 (smooth muscle cells), and MyoFib2 (myofibroblasts), with darker colors indicating higher mean expression levels. (E) The violin plot presents the expression levels of the PRRX1(+) regulon across the mesenchymal stromal cell subsets, revealing significantly higher expression in the MyoFib2 population compared to all other cell types.
