Table 1 The interaction impacts of the soil application of biochar × foliar-spraying of chitosan under varying soil moisture levels on the biochemical, phytochemical, and yield characteristics of yarrow during two years of study.

From: Soil-based biochar and foliar-spraying of chitosan enhances the phytochemical traits of yarrow (Achillea millefolium L.) under varying moisture levels

Irrigation

Biochar (kg/m2 soil)

Chitosan (g/L)

Characteristics

Proline (μmol/g wet weight)

Peroxidase (μmol/mg protein/min)

Ascorbate peroxidase (μmol/mg protein/min)

Catalase (μmol/mg protein/min)

DPPH antioxidant capacity (%)

Biological yield (kg/ha)

Dry flower weight (kg/ha)

Essential oil content (% v/w)

Essential oil yield (L/ha)

Irrigation (80–85% F.C.)

0

0

14.4 k¶

5.45 g

30.75 h

5.96 h

30.75 h

7714.4c

1161.6c

0.29i

3.52d

2.5

19.08i

5.93f.

32.56 h

7.31f.

32.56 h

8615.1b

1259.4b

0.31 h

3.97c

2

0

16.66j

5.59 g

31.19 h

6.7 g

31.19 h

8899.2b

1296.4ab

0.32 g

4.2b

2.5

21.45 h

6.3e

35.78 g

7.61ef

35.78 g

9197.7a

1323.3a

0.33 fg

4.39a

Irrigation (60–65% F.C.)

0

0

23.72 g

6.32e

38.59f.

7.95e

38.59f.

5660.7 g

805.4e

0.34f.

2.47 g

2.5

26.5f.

6.87d

44.06e

8.97d

44.06e

6602.7e

921.5d

0.35e

3.25e

2

0

25.21 fg

6.7d

40.26f.

8.67d

40.26f.

6050.6f.

841e

0.35e

2.99f.

2.5

32.8e

7.15c

46.75d

9.47c

46.75d

7182.6d

948.3d

0.38d

3.64d

Irrigation (40–45% F.C.)

0

0

37.11d

7.45b

53.66c

9.81c

53.66c

4135.9j

609.2 h

0.40c

2.46 h

2.5

46.92b

7.85a

61.75b

10.71b

61.75b

4652.9i

677.7 g

0.42b

2.79 g

2

0

40.28d

7.82a

60b

10.65b

60b

4458.2ij

648.6 g

0.43b

2.68 g

2.5

49.07a

7.72a

65.38a

12.51a

65.38a

5191.6 h

745.9f.

0.44a

3.29e

P-value

0.000**

0.01*

0.000**

0.000**

0.01*

0.03*

0.04*

0.00**

0.01*

  1. ¶ Different letters within a column indicate significant difference at p < 0.05 according to Duncan’s test.
  2. *: significant at the 5% level and **: significant at the 1% level.