Fig. 1
Time-course analysis of osteoclast formation in the mouse model of orthodontic tooth movement (OTM). a Left image: schematic representation of the experimental method in the mouse model of OTM. A Ni-Ti coil spring is ligated to the maxillary left first molar (M1) and the hole near the incisors. Right image: hematoxylin and eosin (H&E) staining of the sagittal section of the maxillary left molars on day 6 after OTM. Scale bar = 200 μm. b Representative images of maxillary left molars and dental impressions on day 0, 6, and 12 after OTM. c Distance of tooth movement from day 0 to day 12 (n = 6). d Tartrate-resistant acid phosphatase (TRAP) staining of horizontal sections with periodontal tissue and distobuccal root of the maxillary left first molar after OTM in wild-type (WT) mice. Scale bar = 100 μm. e Quantification of TRAP-positive cells on the compression side after OTM (n = 6). Black arrows indicate the direction of force. M1, first molar; M2, second molar; M3, third molar; M, mesial side; D, distal side; a, alveolar bone; p, periodontal ligament; r, root. **p < 0.01, ***p < 0.001, ****p < 0.0001.
