Fig. 5

RBM47 regulates alternative splicing of cell cycle, proliferation and apoptosis associated genes in HCT116 cells. (A) Scatter plot exhibiting the most enriched GO biological process results of the RASGs. (B) Scatter plot exhibiting all the proliferative pathways GO biological process results of the RASGs. (C) Scatter plot exhibiting all the apoptotic pathways GO biological process results of the RASGs. (D) Scatter plot exhibiting all the cycle pathways GO biological process results of the RASGs. (E) Hierarchical clustering heat map showing expression levels of all associated genes about (B), (C) and (D) in RBM47 regulated alternative splicing events (RASEs). (F) RBM47 regulates alternative splicing of CD44. Left panel: IGV-sashimi plot showing the regulated alternative splicing events and binding sites across mRNA. Reads distribution of RASE is plotted in the up panel and the transcripts of each gene are shown below. Right panel: The schematic diagrams depict the structures of ASEs. RNA-seq validation of RASEs was shown at the bottom of the right panel. Error bars represent mean ± SEM. *** P-value < 0.001, ** P-value < 0.01, * P-value < 0.05.