Fig. 3
From: Surface keratin 1, a tumor-selective peptide target in human triple-negative breast cancer
K1 levels are significantly increased in human TNBC compared to normal breast and other breast cancer subtypes. (a) Representative breast tissue images showing IHC staining for K1. IHC analysis of human breast tissue array (TissueArray.com) was performed using rabbit monoclonal [EPR17744] to cytokeratin 1 (Abcam) and goat anti-rabbit IgG H&L (HRP polymer) secondary antibody (Abcam). Images of tissues from two patients each with TNBC, ER + /PR +, HER2 + or normal breast are shown (scale bar 50 μm). (b) IHC images of a full TNBC tissue core (scale bar 200 μm) and a 10X magnified region of the tissue. (c) Data for human tissues (from TissueArray.com) used for the IHC images shown above. IC invasive carcinoma (d) Quantification of K1 staining (dark brown) determined as immunoreactive score (IRS) from IHC images of tissues from normal breast (n = 8), HER2 + (n = 14), ER + /PR + (n = 20), and TNBC (n = 24) breast cancer subtypes (refer to Fig. S1 for patient details). Mean ± SEM; ns not significant; ***p < 0.001, Welch’s one-way ANOVA followed by Dunnett’s T3 post-hoc test. (e) Quantification of K1 staining (IRS) of grade 2 (n = 12) and grade 3 (n = 10) TNBC tissues compared to normal breast (n = 8) tissues. Mean ± SEM; *p < 0.05, **p < 0.01, Welch’s one-way ANOVA, followed by Dunnett’s T3 post-hoc test.
