Fig. 5
From: Surface keratin 1, a tumor-selective peptide target in human triple-negative breast cancer
Immunofluorescence analysis of CSK1 expression (green and orange) in human TNBC cells (MDA-MB-231, MDA-MB-436, and MDA-MB-468). (a) Z-section confocal microscopy images of a few representative cells show higher CSK1 expression in TNBC cells compared to the normal mammary epithelial cells (MCF-10A and MCF-12A). Cells were stained with anti-K1 mAb (green), cell-membrane stain (red), and the overlay is shown in orange. DAPI (blue) was used to stain the nucleus. Scale bar 15 μm. (b) Fluorescence intensity of CSK1 (% of cell membrane stain intensity) for TNBC and normal cells. Mean ± SEM; ***p < 0.001 (c) Representative Z-section confocal microscopy images show overlay (orange) of K1 and cell-membrane staining in a large number of TNBC cells. Scale bar 25 μm.
