Fig. 6
From: Surface keratin 1, a tumor-selective peptide target in human triple-negative breast cancer
CSK1 receptor-mediated endocytosis of peptide 18–4 by TNBC cells. Cells (MDA-MB-231 and MDA-MB-468) were incubated with FITC labeled peptide 18–4 (green) in the absence (-) and presence (+) of K1 mAb, followed by incubation with LysoView 640 (orange) for labeling lysosomes. Nuclei were stained with DAPI (blue). (a) Confocal Z-section images of representative cells along with overlay (yellow) are shown. Scale bar 20 μm. (b) Fluorescence intensity of FITC-peptide (% of lysosomes intensity) in TNBC cells in the absence and presence of K1 mAb. Mean ± SEM; ****p < 0.0001.
