Fig. 2

Degradation of xyloglucan and (1→3)(1→4)-β-glucan with TmCel74. Xyloglucan was degraded by TmCel74, and the reaction products were analyzed by HPLC (A) and MALDI-TOF mass spectrometry (C). (1→3)(1→4)-β-glucan was degraded by TmCel74 and the reaction products were analyzed by HPLC (B) and MALDI-TOF mass spectrometry (D). Hex: hexose (glucose or galactose) residue, Glc: glucopyranosyl residue, Xyl: xylopyranosyl residue. Xyloglucan was incubated with TmCel74 in the presence of TmAxy31 (E) or TmBgalB (F). Xyl: xylose, Gal: galactose.