Fig. 4

Substrate specificity of TmBgalB. (A) TmBgalB was incubated with lactose, xyloglucan, xyloglucan oligosaccharides, or pNP β-d-galactopyranoside and the reaction products were analyzed using thin-layer chromatography. A xyloglucan oligosaccharide mixture containing XXXG, XXLG/XLXG, and XLLG (B) was incubated with TmBgalB in the absence (C) or presence (D) of TmAxy31 and the mono- and oligosaccharides produced were analyzed using HPLC.