Fig. 6 | Scientific Reports

Fig. 6

From: Characterization of glycoside hydrolases involved in xyloglucan degradation in the thermophilic bacterium Thermotoga maritima

Fig. 6

Sequence alignments of GH74 and GH31 enzymes. (A) Amino acid sequence alignment of GH74 xyloglucanases. GenBank accession numbers are as follows: Paenibacillus sp. XEG74 (Paenibacillus_XEG74, BAE44527), Clostridium saccharoperbutylacetonicum Xeg74A (Clostridium_Xeg74A, AGF55626), Thermotoga maritima Cel74 (TmCel74, AAD35393), Streptomyces avermitilis SaGH74B (Streptomyces_SaGH74B, BAC70285), and Geotrichum sp. XEG (Geotrichum_XEG, BAD11543). Protein Data Bank (PDB, https://www.rcsb.org) code of Geotrichum sp. XEG is 3A0 F. Amino acid residues in + 3 and + 5 subsites are surrounded. (B) Amino acid sequence alignment of GH31 α-xylosidases. GenBank accession numbers are follows: T. maritima Axy31 (TmAxy31, AAD35396), E. coli YicI (EcYicI, AAC76680), Cellvibrio japonicus Xyl31A (CjXyl31A, ACE86259), and Bacteroides ovatus GH31 (BoGH31, EDO11437). PDB codes of E. coli YicI and C. japonicus Xyl31 A are 1XSI and 2XVG, respectively. PA14 domains are surrounded. Amino acid sequences were aligned using the Clustal Omega program (https://www.ebi.ac.uk/jdispatcher/msa/clustalo). Asterisks, colons, and periods indicate fully conserved, strongly conserved, and weakly conserved amino acid residues, respectively.

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