Fig. 2
Growth of M. maripaludis and M. ruminantum in the presence and absence of the methanogen inhibitor BES or the seaweed derived MWCO fractions added at a concentration of 1 mg/mL (100 µL) observed over a period of 11 days at an absorbance OD405 using a 96 well plate reader. (a) Growth of M. maripaludis reached an OD405 of 0.35 (day 8). In the presence of the tests and inhibitors, growth reached a maximum of 0.03 (day 8). (b) Growth of M. ruminantium reached an OD405 of 0.52 (day 8). In the presence of the tests and inhibitors, growth reached a maximum of 0.02 (day 8). Two-way ANOVA confirmed significant differences, where p < 0.05; p value: 0.00234 (F > F crict ~ 4.041 > 2.266) for M. maripaludis and 6.02128E-13 (F > F crict ~ 19.246 > 2.003) for M. ruminantum.
