Table 6 Formulation of starter culture using design expert software.

From: Production of improved Ethiopian Tej using mixed lactic acid bacteria and yeast starter cultures

Formulates

C 1

C 2

C 3

C 4

C 5

C 6

C 7

C 8

Total volume of starter inoculants

A: AAUL2

B: AAUL6

C: AAUL7

D: AAUL8

E: AAUY4

F: AAUY6

G: AAUY2

H: AAUY5

ml

ml

ml

ml

ml

ml

ml

ml

 

F 1

2.5

0

0

0

2.5

0

0

0

5%

F 2

0

0

2.5

0

0

0

0

2.5

5%

F 3

0.3125

0.3125

0.3125

0.3125

0.3125

2.8125

0.3125

0.3125

5%

F 4

0

0

0

2.5

0

0

2.5

0

5%

F 5

0

2.5

0

0

0

2.5

0

5

5%

F6

0

0

5

0

0

0

0

0

5%

F7

5

0

0

0

0

0

0

0

5%

F8

0

5

0

0

0

0

0

0

5%

Control

 

No starter

  1. NB: C, components generated by the Design expert software; F, possible formulations. Only six of the 8 combinations were used as some of them contain either the LAB or the yeast. Tej samples prepared using potential starter cultures. AAU, Addis Ababa University; L, lactic acid bacteria isolates; Y, yeast isolates and the numbers next to the letters represent the isolate number. Means with different superscript letters in the same column are statistically significant at p < 0.05.